3/9/2024 0 Comments Plit channels imagej fiji![]() If you don't, go back and read about: What is the PSF? Why is it important in colocalization analysis? (for instance: read the Costes paper ).You should know approximately how big the PSF is (in pixels) in your images.Do, at the very very least, 10 iterations (100 would be better).) the larger the number, the longer the analysis will take. Also choose the approximate size of the point spread function (PSF) in your images, as well as the number of iterations to run the Costes statistical significance test (We suggest a large number.Turn the options on/off by clicking the selection button at the left of the Algorithm description.Numerical results and image names are dumped into the ImageJ Log window as comma separated values, so you can copy paste, or save the log window contents, to then import them into whatever statistical package or spreadsheet in which you wish to analyze the results.Choose which "Algorithms" are run and which statistics you wish to calculate, and if you want to save the "standardized" PDF result file, when the OK button is pressed. ![]()
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